OPTIMIZATION OF IN VITRO CALLUS FORMATION AND REGENERATION IN POTATO (SOLANUM TUBEROSUM L., CV. ARIZONA) USING COPPER OXIDE NANOPARTICLES
DOI:
https://doi.org/10.65164/vc170516Abstrak
Potato (Solanum tuberosum L.) is a globally important staple crop, providing essential
carbohydrates and nutrients. In vitro regeneration via callus induction is a critical step in genetic
transformation, tissue culture propagation, and crop improvement programs. Recent studies have
demonstrated that nanoparticles can modulate plant tissue culture processes by influencing cellular
metabolism, nutrient availability, and oxidative stress [1]. This study evaluates the effect of copper
oxide nanoparticles (CuO NPs) on callus induction and regeneration efficiency in potato cv. Arizona.
To assess their impact, different concentrations of CuO NPs were incorporated into Murashige and
Skoog (MS) medium, and their effects on callus formation frequency, growth rate, and subsequent
shoot regeneration were quantified. Low concentrations of CuO NPs significantly promoted callus
induction and shoot regeneration relative to the control, indicating a potential role of CuO NPs in
enhancing in vitro culture efficiency. These findings provide a basis for integrating nanotechnology
into potato tissue culture protocols to accelerate crop improvement programs.
Havolalar
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